Description
Intended Uses: The ELISA Bordetella pertussis toxin IgA and IgG tests are qualitative immunoassays for the demonstration of human antibodies in serum or plasma directed against Bordetella pertussis toxin. The se assays can be used to demonstrate contact with the pathogen and are recommended to be used in combination with the Bordetella pertussis IgA, IgG, and IgM ELISA tests (Codes MBS490254, MBS490256, MBS490258) in various kinds of samples.
Principle of the Assay: This ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in various kinds of samples. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate p-nitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically.
Background: Bordetella pertussis belongs to the genus of Bordetella. It is a small coccoid, gram negative bacillus, which occurs world-wide. The attachment of B. pertussis and B. parapertussis to the ciliated cells in the mucosa of the human respiratory tract is mediated by adhesins. An important adhesion protein and an equally important immunogen is the so-called filamentous haemagglutinin (FHA). Colonisation of the respiratory tract and establishment of infection are facilitated by the synergistic action of several virulence factors. An important virulence factor is pertussis toxin (PT)which mediates multiple biological effects such as leukocytosis, lymphocytosis, mitogenity, and increased sensitivity to histamine